Residues of Heat-Labile Enterotoxin Involved in Bacterial Cell Surface Binding
نویسندگان
چکیده
منابع مشابه
Residues of Heat - Labile Enterotoxin Involved in 7 Bacterial Cell
9 Running title: Surface binding mutants of LT 10 11 12 Benjamin Mudrak 1 , Daniel L. Rodriguez 2 , and Meta J. Kuehn 1,2* 13 Departments of Molecular Genetics and Microbiology 1 and Biochemistry 2 14 Duke University Medical Center, Durham, NC 27710 15 16 17 18 19 20 21 * To whom correspondence should be addressed: Tel. 919-684-2545; Fax 919-684-8885; 22 E-mail: [email protected] 23 Copyr...
متن کاملResidues of heat-labile enterotoxin involved in bacterial cell surface binding.
Enterotoxigenic Escherichia coli (ETEC) is a leading cause of traveler's diarrhea worldwide. One major virulence factor released by this pathogen is the heat-labile enterotoxin LT, which upsets the balance of electrolytes in the intestine. After export, LT binds to lipopolysaccharide (LPS) on the bacterial surface. Although the residues responsible for LT's binding to its host receptor are know...
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Enterotoxigenic Escherichia coli (ETEC) is a significant source of morbidity and mortality worldwide. One major virulence factor released by ETEC is the heat-labile enterotoxin LT, which is structurally and functionally similar to cholera toxin. LT consists of five B subunits carrying a single catalytically active A subunit. LTB binds the monosialoganglioside G(M1), the toxin's host receptor, b...
متن کاملHeat - labile Enterotoxin of Escherichia coli
Heat-labile enterotoxin (LT) was obtained in large quantities (several-gram amounts) and great purity from Escherichia coli C600 carrying the LT-coding multicopy plasmid EWD299. By growing this strain on a medium that allows high cell densities in the early stationary phase, we increased the net LT production per milliliter by a factor of 200, compared to natural porcine enterotoxigenic E. coli...
متن کاملEscherichia coli Heat - labile Enterotoxin NUCLEOTIDE SEQUENCE
We report the complete DNA sequence of the Escherichia coli elt A gene, which codes for the A subunit of the heat-labile enterotoxin, LT. The amino acid sequence of the LT A subunit has been deduced from the DNA sequence of elt A. The LT A subunit starts with methionine, ends with leucine, and comprises 264 amino acids. The computed molecular weight of LT A is 29,673. The A subunit of cho...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2009
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.01622-08